Secondary metabolites by chemical screening. 4. Detection, isolation and biological activities of chiral synthons from Streptomyces.

by using special staining reagents on TLCshould result in interesting precursors for alkaloid synthesis. The screening was performed by centrifugation of the culture-broth from different Streptomyces strains, adsorption of the compounds in the culture filtrate on Amberlite XAD-16, elution with methanol and concentration. Detection of the amines after TLC separation of the products was done using phenothiazine perbromide, an excellent reagent for visualization of secondary amines3), which results in blue-colored spots on TLC. At room temperature primary amines were ruled out through a color change after treatment with a sodium